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correlograms displaying pearson's correlation coefficient gene expression in hpciss  (RStudio)
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RStudio correlograms displaying pearson's correlation coefficient gene expression in hpciss
ABCB1 , CYP3A4 , PXR , and RXRA gene expression in hPCIS determined by qRT-PCR. Fold changes in ABCB1 and CYP3A4 mRNA levels in hPCISs incubated with RIF (30 µM) for 4, 8, 12, 16, 20, 24, and 48 h (A) , relative to expression in RIF-free controls, showing that RIF significantly increased mRNA levels of both genes in samples from all donors at all tested time points (one-sample t -tests, three slices from one donor: *, p < 0.05; **, p < 0.01; ***, p < 0.001). PXR (B) and RXRA (C) were demonstrated to be expressed in hPCISs incubated with RIF (30 µM) at all tested time points. Amounts of PXR and RXRA transcripts are presented in arbitrary units (a.u.), calculated as 2 −∆Ct × 10 6 . (D) The matrix of <t>Pearson’s</t> coefficients of correlation was used to assess associations among all targeted transcripts in RIF-treated hPCISs (positive in blue and negative in red, with color intensity proportional to the magnitude of the coefficients). Asterisks indicate significant differences: *, p < 0.05; **, p < 0.01; ***, p < 0.001.
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ABCB1 , CYP3A4 , PXR , and RXRA gene expression in hPCIS determined by qRT-PCR. Fold changes in ABCB1 and CYP3A4 mRNA levels in hPCISs incubated with RIF (30 µM) for 4, 8, 12, 16, 20, 24, and 48 h (A) , relative to expression in RIF-free controls, showing that RIF significantly increased mRNA levels of both genes in samples from all donors at all tested time points (one-sample t -tests, three slices from one donor: *, p < 0.05; **, p < 0.01; ***, p < 0.001). PXR (B) and RXRA (C) were demonstrated to be expressed in hPCISs incubated with RIF (30 µM) at all tested time points. Amounts of PXR and RXRA transcripts are presented in arbitrary units (a.u.), calculated as 2 −∆Ct × 10 6 . (D) The matrix of Pearson’s coefficients of correlation was used to assess associations among all targeted transcripts in RIF-treated hPCISs (positive in blue and negative in red, with color intensity proportional to the magnitude of the coefficients). Asterisks indicate significant differences: *, p < 0.05; **, p < 0.01; ***, p < 0.001.

Journal: Frontiers in Pharmacology

Article Title: Rifampicin Induces Gene, Protein, and Activity of P-Glycoprotein (ABCB1) in Human Precision-Cut Intestinal Slices

doi: 10.3389/fphar.2021.684156

Figure Lengend Snippet: ABCB1 , CYP3A4 , PXR , and RXRA gene expression in hPCIS determined by qRT-PCR. Fold changes in ABCB1 and CYP3A4 mRNA levels in hPCISs incubated with RIF (30 µM) for 4, 8, 12, 16, 20, 24, and 48 h (A) , relative to expression in RIF-free controls, showing that RIF significantly increased mRNA levels of both genes in samples from all donors at all tested time points (one-sample t -tests, three slices from one donor: *, p < 0.05; **, p < 0.01; ***, p < 0.001). PXR (B) and RXRA (C) were demonstrated to be expressed in hPCISs incubated with RIF (30 µM) at all tested time points. Amounts of PXR and RXRA transcripts are presented in arbitrary units (a.u.), calculated as 2 −∆Ct × 10 6 . (D) The matrix of Pearson’s coefficients of correlation was used to assess associations among all targeted transcripts in RIF-treated hPCISs (positive in blue and negative in red, with color intensity proportional to the magnitude of the coefficients). Asterisks indicate significant differences: *, p < 0.05; **, p < 0.01; ***, p < 0.001.

Article Snippet: Correlograms displaying Pearson’s correlation coefficient gene expression in hPCISs (considers all times points, in which gene expressions were analyzed) were generated using R-Studio 1.3.959.

Techniques: Gene Expression, Quantitative RT-PCR, Incubation, Expressing